Description
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human Neutrophil Extracellular traps, NETs present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human Neutrophil Extracellular traps, NETs in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm